Figure 1. P. brasiliensis-infected mice treated with CFA present remarkable decrease of fungal burden and increase of cytokines production.

Mice inoculated with 1×10⁶ yeast cells were treated with CFA (therapy) or IFA (control) on day 20 postinfection. The lung homogenates obtained from these mice on day 30 after treatment were analyzed for CFU (A), IFN-γ (B), IL-4 (C), IL-12 (D), TNF-α (E), IL-10 (F), TGF-β (G). In the same period the levels of P. brasiliensis circulating antigen was analyzed in serum (H). Dashed line in panel A represents the amount of viable yeasts at the day of treatment (20 days postinfection). Data are reported as the mean±standard deviation for three mice per group performed in duplicate. *P<0.05 compared to control group (two-tailed Mann-Whitney U-test).