Figure 5.

Nuclear import is not affected by microinjection of anti-hGle1p antibody. HeLa cells transiently expressing GR-GFP (A, D, G, and J) were co-microinjected with Texas red–dextran and either affinity-purified rabbit antibody: anti-hGle1p (2 mg/ml, A–F) or anti-MBP (2 mg/ml, G–L). The anti-MBP antibody served as a control for nonspecific effects of antibody injection. Texas red–dextran (Center, B, E, H, and K) differentiates between injected and uninjected cells. In a given field, only a subset of the cells were cytoplasmically injected. Cells were incubated for 12 hr at 37°C, and those in D–F and J–L were treated with medium containing 10 μg/ml dexamethasone for 30 min before fixation and processing for fluorescence microscopy. Cells in A–C and G–I were untreated. Nuclear DNA was stained with 4′,6-diamidino-2-phenylindole (C, F, I, and L). (Bar = 20 μm.)