Figure 3.

Both Wee1 and CDC27 are substrates of the caspase-3 like protease. (A) Inhibition of Wee1 and CDC27 cleavage by DEVD-CHO; 2 × 10⁶ Jurkat cells were treated with 10 ng/ml anti-Fas antibody for 4 hr in the presence of various concentrations of DEVD-CHO. The percentage of cell death was measured by MTT assay (20). Cleavages of CDC27 and Wee1 were examined by Western blotting by using anti-CDC27 and anti-Wee1 antibodies. (B) Cleavages of in vitro translated, ³⁵S-labeled Wee1 and CDC 27 by various caspases. Lanes: 1, inactive caspase; 2, caspase-3; 3, caspase-1; 4, caspase-2; 5, caspase-7; and 6, caspase-8. The arrows indicate the cleavage products.