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. 1998 Jun 9;95(12):6785–6790. doi: 10.1073/pnas.95.12.6785

Figure 4.

Figure 4

Changes in APC activity and cyclin levels during apoptosis. (A) APC activity assay. Lanes: 1, APC from control Jurkat cell extract; 2, APC from apoptotic Jurkat cell extract (88% cell death); 3, purified Xenopus interphase APC with mock treatment; 4, purified Xenopus interphase APC treated with caspase-3; 5, purified Xenopus mitotic APC with mock treatment; and 6, purified Xenopus mitotic APC treated with caspase-3. (B) Samples used for ubiquitin ligase activity were analyzed by anti-CDC27 immunoblotting. The arrows indicate the cleavage products of CDC27. Lane description is the same as in A. (C) Relative levels of cyclin A (□) and cyclin B (•) during the time course of Fas-induced apoptosis, as measured by immunoblotting and quantitated by imagequant program.