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. 1998 Jun 9;95(12):6785–6790. doi: 10.1073/pnas.95.12.6785

Table 1.

H1 kinase activities of various immunoprecipitations from Jurkat cell extracts

Treatment H1 kinase activity
cdc2 cdk2 Cyclin A Cyclin B
−αFas 15 ± 6 24 ± 5 27 ± 6 4 ± 2
+αFas* 97 ± 22 51 ± 9 110 ± 27 10.1 ± 2
Nocadazole arrested 91 ± 21 60 ± 10 121 ± 32 275 ± 53

Activities are reported in units of picomol of Pi per 30 min per milligram of cell extract at room temperature and presented as mean ± SD of triplicate determinations. 

*

Jurkat cells were treated with 10 ng/ml anti-Fas (7C11) for 6 hr, and the cell death percentage was 91% measured by MTT assay (20). 

Approximately 50% cells in mitosis as indicated by FACS analysis.