Table 1.
Treatment | H1 kinase activity
|
|||
---|---|---|---|---|
cdc2 | cdk2 | Cyclin A | Cyclin B | |
−αFas | 15 ± 6 | 24 ± 5 | 27 ± 6 | 4 ± 2 |
+αFas* | 97 ± 22 | 51 ± 9 | 110 ± 27 | 10.1 ± 2 |
Nocadazole arrested† | 91 ± 21 | 60 ± 10 | 121 ± 32 | 275 ± 53 |
Activities are reported in units of picomol of Pi per 30 min per milligram of cell extract at room temperature and presented as mean ± SD of triplicate determinations.
Jurkat cells were treated with 10 ng/ml anti-Fas (7C11) for 6 hr, and the cell death percentage was 91% measured by MTT assay (20).
Approximately 50% cells in mitosis as indicated by FACS analysis.