Figure 4.
Arachidonic acid and Ang II induced tyrosine phosphorylation of Shc in renal proximal epithelial cells. The cells were stimulated with either 20 μM arachidonic acid or 1 μM Ang II for the indicated times. Cell lysates were immunoprecipitated with an anti-Shc polyclonal antibody. The immunoprecipitates were analyzed by SDS/PAGE and immunoblotting by using antibodies to phosphotyrosine (A) and Shc (B) as indicated. I.P., immunoprecipitation; ptyr, phosphotyrosine.