Figure 6.

Arachidonic acid and Ang II induced association of Shc with p21ras in renal proximal tubular epithelial cells. The cells were stimulated with either 20 μM arachidonic acid or 1 μM Ang II for the indicated times. The cell lysates were immunoprecipitated with an anti-ras antibody. The immunoprecipitates were analyzed by SDS/PAGE and immunoblotting used antibodies to Shc (A), and ras (B). The positions of Shc and ras are indicated on the left. I.P., immunoprecipitation; ptyr, phosphotyrosine.