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. 1998 Jun 9;95(12):6791–6796. doi: 10.1073/pnas.95.12.6791

Figure 1.

Figure 1

NaBu and TSA induce p21 mRNA expression in HT-29 cells in an immediate-early fashion. (A) Dose response for p21 induction by NaBu. HT-29 cells were treated with various concentration of NaBu, 0–20 mM for 24 hr, and p21 mRNA expression was examined by Northern blot analyses. (B) Time course for induction of p21 by NaBu. Cells were treated with 5 mM NaBu for varying lengths of time, from 0 to 24 hr, and p21 induction was examined by Northern analysis. (C) p21 induction by NaBu is not blocked by protein synthesis inhibition. HT-29 cells were treated concomitantly with 5 mM NaBu and the protein synthesis inhibitor, cycloheximide (CHX, 10 μg/ml), for 48 hr. (D) Time course for p21 induction by TSA. Cells were treated with 0.3 μM TSA for varying lengths of time. (E) p21 induction by TSA is not blocked by protein synthesis inhibition. Cells were treated concomitantly with 0.3 μM TSA and 10 μg/ml CHX for 6 hr. Representative Northern blots (n = 4) are depicted in each figure. The actin control is shown in the lower panel. Twenty micrograms of total RNA was loaded in each lane, with equal loading verified by ethidium bromide staining.