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. 1998 Jun 9;95(12):6791–6796. doi: 10.1073/pnas.95.12.6791

Figure 2.

Figure 2

Overexpression of histone deacetylase (HDAC1) blocks induction of the p21 promoter by NaBu and TSA. (A) NaBu and TSA induce p21 promoter activity. HT-29 cells were transiently transfected with CAT reporter plasmids under the control of various amounts of the mouse p21 promoter (0–4.7 kb upstream from the transcriptional start site). Transfectants were treated without or with 5 mM NaBu or 0.3 μM TSA, and total cellular protein was examined for CAT expression. Results are shown for the 0-kb (pCAT), 1.4-kb (p211.4CAT), and 4.7-kb (p214.7CAT) p21 promoter constructs and are expressed as fold CAT induction compared with the untreated negative control, arbitrarily taken as 1. (B) Cotransfection of HDAC1 blocks p21 promoter induction by NaBu and TSA. Transfections were performed with the p214.7 CAT −/+ HDAC1, and cells were treated without or with 5 mM NaBu or 0.3 μM TSA. The results are expressed as fold CAT induction compared with the untreated negative control (arbitrarily taken as 1). All results are shown as mean ± SEM (n ≥ 4, in all cases).