Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Yrb2p does not shuttle between the nucleus and the cytoplasm. The nup49–313 strain expressing GFP-Yrb2p or GFP-Npl3p was used for the protein-shuttling assay. After expression/repression at 25°C, cells were shifted to 37°C and incubated for 5.5 hr and the fusion proteins were localized by fluorescence microscopy.
