Figure 4.

Natural lengths of microvilli (A) and extended lengths of microvilli (B). Error bars stand for their SDs. The measurements for anti-CD162 are drawn intentionally at a pressure 0.02 pN/μm² less than the actual pressure so that their error bars can be differentiated from the ones for anti-CD45. (A) The lack of dependence of the free motion rebound length (L₀) upon the suction pressure. L₀ is considered to be the natural length of a microvillus and is directly measured from the tracking data as shown in Fig. 3A and Fig. 3B. The solid circle shows the average for all the measurements. The number of measurements at each pressure is 7, 8, 14, 15, 9, 9, and 9 (left to right). The number of measurements for anti-CD162 at each of the three lower pressures is six. (B) The dependence of the extended length (ΔL) upon the suction pressure. ΔL is obtained by fitting L = L_∞ − ΔLe^−t/t_c to the diminishing rebound of the extension curve shown in Fig. 3A. The number of measurements for anti-CD45 at each pressure is 7, 8, 14, 11, 6, 5, and 1 (left to right). The number of measurements for anti-CD162 at each of the three lower pressures is six.