Skip to main content
. 1998 Jun 23;95(13):7439–7444. doi: 10.1073/pnas.95.13.7439

Figure 6.

Figure 6

MEK inhibition blocks Bad phosphorylation without effect on survival. (A) Cells were preincubated with PD98059 (PD) at the indicated concentrations for 30 min followed by stimulation with IL-3 or GM-CSF for 5 min. Detergent solubilized cell lysates were fractionated by SDS/PAGE and immunoblotted with anti-phospho-MAPK. (B) Bad was immunoprecipitated from the same cells lysates and immunoblotted to determine mobility shift. These results are representative of three independent experiments. (C) MC/9 cells were washed and resuspended in IL-3, GM-CSF, or media alone (Unstim) and incubated with 50 μM PD98059 (solid bars) or vehicle (shaded bars) for 11 hr. Apoptosis was determined by annexin V binding by using flow cytometry. Results shown are averages of duplicate samples and are representative of two independent experiments.