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. 1998 Jun 9;95(12):6809–6814. doi: 10.1073/pnas.95.12.6809

Figure 4.

Figure 4

The various EGF-like ligands induce differential tyrosine phosphorylation and MAPK activation in the 32D.E4 and 32D.E2/D4 transfectants. (A) Detection of EGF-like ligand-induced tyrosine phosphorylation of cellular proteins in 32D.E4 and 32D.E2/E4 cells. Lysates from untreated cells (Control) or cells treated with EGF, NRG1-β, BTC, TGF-α, HB-EGF, or AR were immunoprecipitated (IP) and subsequently immunoblotted (Blot) with anti-pTyr as designated. Molecular mass markers are given in kDa. (B) The effects of EGF-like ligand treatment on the potency and duration of MAPK activation in 32D.E4 and 32D.E2/E4 cells. Lysates from untreated cells (O) or cells treated with the various EGF-like ligands for 5, 10, 30, or 60 min were subjected to immunoblot analysis with an antibody that specifically recognizes the activated forms of Erk1 and Erk2 marked by arrows.