Table 1.
Effects of EGF-like ligands on adherence and monocytic differentiation markers in the 32D transfectants
| Ligand | Adherence*
|
Mac1†
|
FcγRII/III†
|
|||
|---|---|---|---|---|---|---|
| E4‡ | E2/E4‡ | E4‡ | E2/E4‡ | E4‡ | E2/E4‡ | |
| 0 | – | – | 10.6 | 12.9 | 35.0 | 33.3 |
| EGF | – | +++ | 12.1 | 49.2 | 37.0 | 107.0 |
| NRG1-β | +/− | +++ | 16.0 | 39.1 | 47.6 | 124.6 |
| BTC | +/− | +++ | 17.8 | 40.2 | 52.5 | 144.0 |
| TGF-α | – | ++ | 11.2 | 35.1 | 31.7 | 84.4 |
| HB-EGF | – | – | 11.1 | 12.4 | 33.1 | 39.0 |
*
Adherence was based on the percent of cells that attached to the tissue culture substrate before or after stimulation with ligand (100 ng/ml) for 24 h. −, No adherence; +/−, <10%; +, 10–25%; ++, 25–50%; +++, >50%.
†
The numbers shown represent the mean fluorescence intensity of staining by FITC-conjugated anti-Mac1 and anti-FcγRII/III antibodies as determined by flow cytometry before or after stimulation with ligand (100 ng/ml) for 24 h.
‡
E4 and E2/E4 are the abbreviation of 32D.E4 and 32D.E2/E4, respectively.