Figure 2.

Human CUL1 can interact with human SKP1, SKP2, and Cyclin A/CDK2. Sf9 insect cells were infected with baculovirus constructs that express various human proteins as indicated. Cells were labeled with Tran[³⁵S]-label for 3 hr before harvesting. ^PHis6hCUL1 (A) and CDK2^HA (B) together with associated proteins were immunoprecipitated by anti-Polyoma and anti-HA beads, respectively. The composition of the protein complexes in the immunoprecipitates was analyzed by SDS/PAGE followed by autoradiography.