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. 1998 Jun 9;95(12):6825–6830. doi: 10.1073/pnas.95.12.6825

Figure 1.

Figure 1

The coding and partial noncoding nucleotide sequence of the msCRABP gene and deduced amino acid sequence. Exon and protein sequences are upper case. Intron and 5′ and 3′ regulatory sequences are lower case. Protein sequence is listed in one letter code below the second nucleotide of each codon. Nucleotide and amino acid (aa) numbers are shown to the right of the sequence. The letter in parentheses indicates the amino acid (I23) encoded by a split codon. Nucleotide one is the first nucleotide of exon 1. Negative numbers indicate nucleotide sequence upstream of the transcription initiation site. Positive numbers are for the cDNA sequence only. All identified motifs in the regulatory regions and transcription unit are in boldface. Regulatory regions: Shown with shaded ovals is a RARE-like motif (see text). Underlined are several putative transcription factor binding motifs (GC boxes), four repetitive sequences (labeled a-d), and transcription termination processing signals (GT cluster; 3′ regulatory region). Shown boxed is a CAAT and GAGA/purine-like box, a Drosophila zeste site, and a GATA-1 site. msCRABP transcription unit: the transcription initiation site is boxed and labeled mRNA START. Box with a bent arrow is the initiator codon for translation start and box with a STOP is the termination codon. Shown with an open oval close to transcription initiation is a downstream element. Underlined are putative branchpoint sequences for intron splicing (boldface nucleotides represent consensus), a consensus site for the transcription factor AP2, and two poly(A) addition signals (ATTAAA). Strong polypyrimidine tracts preceding 3′ splice sites (intron 1 and 3) are underscored with dots. The first and last two nucleotides of each intron are in boldface. Circled and labeled poly(A) is the poly(A) addition site. Shown in boldface with bidirectional arrows are two large palindromic sequences in intron 1 and 3. The deduced msCRABP amino acid sequence differs by two amino acids (shown in square boxes) compared with the prothoracicotropic hormone peptide fragment sequence (20), although the two residues in question were determined with low confidence during prothoracicotropic hormone sequencing. The successful degenerate primers are noted by brackets and the cDNA sequence used for Southern hybridization is delimited by large parentheses.