Skip to main content
. Author manuscript; available in PMC: 2009 Mar 14.
Published in final edited form as: Biochem Biophys Res Commun. 2008 Jan 9;367(3):649–655. doi: 10.1016/j.bbrc.2007.12.181

Figure 2.

Figure 2

GRKγ interacts preferentially with inactive Gαs and Gβ. 293T cells were transfected with GRK4γ and either AU1-tagged Gαs (a), or wild-type and constitutively active Gαs (b & c). AU1 tagged Gαs was stimulated with AlF4−. 293T cells were lysed with either a triton X-100 (TX-100) based lysis buffer, a RIPA buffer, or a detergent-free buffer to examine the effects of the lysis buffer (c). Endogenous Gβ is co-immunoprecipitated with GRK4γ by Gαs (d), conversely endogenous Gβ co-immunoprecipitates GRK4γ (e). In all panels the upper blots are the co-immunoprecipitation and the lower blots are the control total cell lysates (TCL). Each experiment was conducted a minimum of two times