Fig. 4.

Repeatability of carbachol-induced outward-current responses. (A) Sequential 30-s applications of carbachol (cch; 100 μM) produced repeated outward-current responses. Top trace is the response produced upon the initial application of carbachol; bottom trace, the response on the fourth application. The interval between carbachol applications is 5 min. (B) Amplitude and/or frequency, latency and duration of sequential responses to carbachol are plotted as a percent of the response to the initial application (trial 1). Carbachol-induced outward-current response features are relatively stable but have a non-significant tendency to decrease in amplitude and total current, and increase in latency with increasing trial number (n=3).