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. Author manuscript; available in PMC: 2008 Nov 1.
Published in final edited form as: Comp Biochem Physiol A Mol Integr Physiol. 2007 Aug 8;148(3):584–590. doi: 10.1016/j.cbpa.2007.08.001

Figure 3.

Figure 3

SDS-PAGE analysis of 3 weeks old pig duodenum and characterization of antibody specificity. Lane 1: protein (18 μg) resolved without beta-mercaptoethanol in the sample buffer; Lane 2: protein (18 μg) resolved with beta-mercaptoethanol in the sample buffer; Lane 3: protein (18 μg) resolved with beta-mercaptoethanol in the sample buffer, and anti-serum (1:1000) incubated with excess antigen (0.8 mg/ml peptide) for 2 h prior to blotting of nitrocellulose membranes with electrophoresed proteins.