Skip to main content
. Author manuscript; available in PMC: 2008 Aug 1.
Published in final edited form as: Immunity. 2008 Jan 31;28(2):183–196. doi: 10.1016/j.immuni.2007.11.024

Figure 6. CD148 and CD45 regulate Fc receptor mediated phagocytosis and cytokine production in BMDMs.

Figure 6

(A) BMDMs were incubated with human red blood cells coated with mouse IgG2a mAb. BMDMs with ingested erythrocytes were fixed, labeled with Hoechst and quantified under fluorescent microscope. Phagocytic index was determined as [number of erythrocytes] / [number of nuclei]. Phagocytic index of WT macrophages was then set as 100%. * p< 0.001 (B,C) BMDMs were stimulated with plate-bound mAb to FcR II/III (2.4G2) (B) or LPS (C) in the presence of brefeldin A for 4 hours. TNFα production was detected by intracellular staining with TNFα-specific antibody. (D) BMDMs were stained with mAb to FcγR II/III (2.4G2) or mouse IgG. The expression levels on the BMDMs of the indicated genotypes over the negative control (shaded histogram) are shown.