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. 2008 Feb 22;105(9):3274–3279. doi: 10.1073/pnas.0712235105

Fig. 2.

Fig. 2.

Both PDCD4 MA3 domains bind to eIF4A-NTD but not to eIF4A-CTD. (A and C). Overlays of [15N,1H]TROSY-HSQC spectra of 15N-labeled MA3-m (150 μM) with increasing amount of nonlabeled eIF4A-NTD (A) or eIF4A-CTD (C). Concentrations of eIF4A-NTD/CTD added were 0 μM (red), 150 μM (MA3-m/eIF4A = 1:1 ratio, purple), and 300 μM (MA3-m/eIF4A = 1:2 ratio, blue). (B and D) Overlays of [1H,15N]TROSY-HSQC spectra of 15N-labeled MA3-c (150 μM) with increasing amount of eIF4A-NTD (B) or eIF4A-CTD (D). Concentrations of 0 μM (red), 150 μM (purple), and 300 μM (blue) are shown. Regions with the most distinct chemical shift changes observed are enlarged in the side box.