Table 1.
Peptide mass fingerprinting of recombinant hPod from yeast cells
| Peptide name | Theoretical m/z [M + H]+ | Observed MALDI-TOF | Expected glycopeptide |
|---|---|---|---|
| TRY-1 from hPod/N-1 | 4,161.22 | 4,177* | |
| TRY-1 from hPod/TN-2 | 4,161.22 | 4,177* | |
| TRY-1 from hPod/TN-3 | 4,161.22 | 4,177* | |
| TRY-1 from hPod/TN-1 | 4,161.22 | 4,177* | |
| 4,380* | TRY-1 with HexNAc | ||
| TRY-1 from hPod/T-1 | 4,161.22 | 4,380* | TRY-1 with HexNAc |
| 4,542* | TRY-1 with one HexNAc and one Hex | ||
| 4,745* | TRY-1 with two HexNAc and one Hex | ||
| 4,907* | TRY-1 with two HexNAc and two Hex |
*TRY-1 possesses one methionine residue, and this residue was oxidized. hPod/TN-1, hPod/T-1, hPod/TN-3, hPod/T-3, and hPod/N-1 show hPod produced by TN-1 cells, T-1 cells, TN-3 cells, T-3 cells, and N-1 cells.