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Generation of TRAMP-reactive CD8+ T cell lines and hybridomas. (A) The tumor specificity of the T cell line was determined by specific IFN-γ production in response to TRAMP-C2, MC38, B16BL6, or EL4 target cells. Standard deviations of triplicate cultures are shown. (B) The tumor specificity of the T cell line was determined by cytotoxicity using a standard JAM assay. T cells were cultured at varied effector to target cell ratios (E:T) with TRAMP-C2 or MC38 EL4 target cells.
