Fig. 2.
Rate and accuracy of mRNA translation in wild-type and mutant mitochondrial hybrid ribosomes. (A) AUG(UUU)12 mRNA-directed incorporation of phenylalanine and leucine into oligopeptides synthesized by ribosomes containing either a wild-type mitochondrial, mutant A1555G, or mutant C1494U decoding site (mean ± SD; n = 3). M. smegmatis wild-type ribosomes are included for comparison. For leucine per phenylalanine ratios, see Table 1. (B) Relative bioluminescence in luciferase synthesis assays (■) and total leucine incorporation (□) by mitochondrial hybrid and mutant A1555G ribosomes (mean ± SD; n = 3). Bacterial wild-type ribosomes are included for comparison. For luciferase activity per amino acid incorporation ratios, see Table 2.