Table 1.

Capacity and accuracy of amino acid incorporation

Decoding site	AUG(UUU)12-directed amino acid incorporation
	Phe, pmol	Leu, pmol	Leu/Phe
M. smegmatis	31	1.3	0.04
Mitochondrial	25	1.2	0.05
A1555G	29	8.5	0.29
C1494U	33	4.4	0.13
C1556G	29	0.7	0.03

Amino acid incorporation is given as the mean amount of [¹⁴C]phenylalanine and [³H]leucine incorporated by 5 pmol of purified ribosomes after 60-min incubation with AUG(UUU)₁₂ mRNA as template (n = 3). See Fig. 2A and SI Fig. 7 for corresponding time curves and standard deviations.