Fig. 5.

Genome-wide expression analysis of genes regulated by RFL. (A) Comparison of fold change in expression levels in wild-type versus dsRNAiRFL panicles for nine representative transcripts chosen from microarray data (SI Table 3). Data from microarrays are compared with that from quantitative RT-PCR analysis. (B–I) In situ RNA hybridization of an RFL-regulated transcript, AK101504 (PIN3-like). Transcripts at leaf axils (B, green arrow) and in a young tiller bud (C, cyan arrow) are indicated. (D) Transcripts in the panicle apex (purple arrowhead) and initiating primary branch (red arrowhead). (E) Expression at the apical end of a primary branch (red arrowhead) and in emerging secondary branches (white arrowhead). (F) Uniform expression in a young spikelet meristem (yellow arrowhead). (G) Transcripts in the emerging lemma (black arrow), palea (red arrow), and carpel anlagen (blue arrowhead) of spikelets with differentiating organs. (H) Expression in the vascular strands of an emerging primary branch (pink arrow). (I) Panicle probed with sense AK101504 RNA. (Scale bars: B–D, F, and I, 20 μm; E and G, 50 μm; H, 10 μm.)