Fig. 2.

Notch induces the immunosuppressive cytokine IL-10 in established IFN-γ-secreting Th1 cells. IFN-γ producers that had been isolated from 1-week-old Th1 cells were subjected to an additional week of Th1 culture and retroviral transduction with Notch (N) or GFP alone as a control (C). After PMA/ionomycin restimulation, IFN-γ and IL-10 production was analyzed by intracellular staining and in culture supernatants. (Data are representative of two independent experiments.)