Fig. 3.

Notch-mediated IL-10 induction strictly depends on STAT4 activation. (A) Analysis of IL-10 production by intracellular staining after PMA/ionomycin restimulation of naïve T cells transduced with active Notch or GFP alone as control and cultured for 5 days cultured under Th1 conditions and plate-bound anti-CD3/anti-CD28 stimulation. (B) IL-10 levels in culture supernatants of PMA/ionomycin-restimulated isolated Notch- or control-transduced T cells from wild-type, STAT4^−/−, or IFN-γR^−/− mice that had been cultured for 5 days under Th1 conditions. (C) Expression of GATA-3 and T-bet in isolated Notch (N)- or control (C)-transduced T cells after 5 days of culture under Th1 conditions. (D and E) IL-10 levels in culture supernatants of PMA/ionomycin-restimulated isolated Notch- or control-transduced T cells from wild-type and T-bet^−/− mice that had been cultured for 5 days under Th1 conditions (D) or from wild-type and STAT4^−/− mice that had been cultured for 5 days in the presence of IL-27 (E). (Data are representative of at least two independent experiments.)