Fig. 5.

CpG induces Notch-dependent IL-10 production in Th1 cells in vitro and in vivo. (A) IL-10 and IFN-γ expression analyzed by intracellular staining of naïve T cells after 5 days of coculture with CD11c⁺ DCs from spleen and lymph nodes and addition of IL-12, CpG, or IL-12/CpG in the presence or absence of GSI (gated on CD4⁺ T cells). (Data are representative of at least three independent experiments.) (B) IL-10 and IFN-γ expression analyzed by intracellular staining after PMA/ionomycin restimulation of OVA-TCR transgenic T cells that had been isolated from draining lymph nodes after adoptive transfer and s.c. immunization with OVA/CpG in the presence or absence of GSI. (Data are representative of three independent experiments, three animals per group.)