Fig. 4.

Shear stress alters epithelial transporter expression. (A) In NHBE cells, shear stress decreased AQP5 abundance and increased αENaC expression with no change in αNa, K-ATPase expression. (B) NHBE cells treated with inhibitor were exposed to shear stress for 2 h. TRPV4 inhibition with 20 μM RR and VGCC inhibition with 10 μM verapamil or 10 μM nifedipine blocked shear-induced decreases in AQP5; the TRPV1 inhibitor, capsazepine (50 μM), had no effect. (C) In NHBE cells, under static conditions, TRPV4 activation with 4αPDD decreases AQP5, which was blocked by VGCC inhibition with nifedipine. VGCC activation with BayK-8644 decreased AQP5; the TRPV4 inhibitor, RR, did not prevent the decrease (n = 3). *, P < 0.05 with ANOVA one-way Bonferroni correction.