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. Author manuscript; available in PMC: 2008 Dec 14.
Published in final edited form as: Brain Res. 2007 Sep 22;1185:256–265. doi: 10.1016/j.brainres.2007.09.034

Figure 1. AAV2 IGF-1 delivery results in long-term IGF-1 expression and rescues motor neurons in the lumbar spinal cord of SOD1G93A mice.

Figure 1

IGF-1 (A, C) and GFP (D) were expressed in SOD1G93A lumbar cord at high levels even at disease end-stage. IGF-1 (C - arrows: enlargement of box in A) and GFP (D - inset) expression was specifically noted in ventral horn motor neurons. Expression of human IGF-1 was not seen in formulation buffer-injected cords (B: Insert is higher magnification enlargement of box). Co-localization of GFP with GFAP+ astrocytes was not observed (E). AAV2 GFP and AAV2 IGF-1 SOD1G93A mice had greatly reduced numbers of motor neurons compared to wild-type age-matched mice at 110 days of age (F). Compared to AAV2 GFP injected controls, AAV2 IGF-1 mice had a significantly greater number of motor neurons at 110 days of age (F). No differences in the response of Iba1+ microglia was noted between lumbar spinal cord of AAV2 GFP and AAV2 IGF-1 mice (G–H)