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. Author manuscript; available in PMC: 2008 Mar 6.
Published in final edited form as: J Neurochem. 2007 Nov 13;104(5):1233–1248. doi: 10.1111/j.1471-4159.2007.05054.x

Fig. 7.

Fig. 7

Replacement of the GC-box with a single Gal4 binding element and challenge with Gal4 fusion proteins supports a role for Sp3 in basal transcription. (a) A schematic diagram of the Gal4-0.27GCH1-GL3 luciferase reporter construct in which the 22 bp GC-box has been replaced with a single 19 bp Gal4 cis-element. Fusion proteins of the Gal4 DNA binding domain and the activation domains of Sp1, Sp3, and C/EBPβ are also shown. Luciferase assays of PC12 cells transiently transfected with 420 ng of Gal4-0.27GCH1-GL3, 40 ng of pRL-null, 150 ng Gal4-0, Gal4-Sp1, Gal4-Sp3 or Gal4-C/EBPβ or carrier plasmid DNA and then incubated with 5 mmol/L 8Br-cAMP (cAMP) for 4 h. The insert shows control activity plotted on a smaller scale. Data were analyzed by ANOVA with post hoc Bonferroni tests (*p < 0.05 vs. Gal4-0 control). (b) A schematic diagram of the 5XGal4-heterologous minimal luciferase reporter pFR-luc. Luciferase assays of PC12 cells transiently transfected with 420 ng 5XGal4-heterologous minimal luciferase reporter, 40 ng pRL-null, 150 ng Gal4-0, Gal4-Sp1 or Gal4-Sp3 and carrier plasmid DNA. Data were analyzed by ANOVA with post hoc Bonferroni tests (*p < 0.05 vs. Gal4-0).