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. 2008 Jan 3;9:1. doi: 10.1186/1471-2091-9-1

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Copyright © 2008 Menon et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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SAP130 forms tertiary complexes with CRL substrate adaptors and is associated with E3 activity. (A) HEK293 cells were transfected with HA-VHL or Flag-SAP130. Anti-HA and anti-Flag IP samples were blotted with indicated antibodies. An asterisk (*) indicates a non-specific band in Flag IP. (B) HA-SAP130 was expressed in HEK293 cells. Anti-HA IP samples were probed with indicated antibodies. (C) Flag immunocomplex isolated from HEK293 cells expressing empty vector, Flag-SAP130 or Flag-CPSF160 were incubated with N-terminal biotin-labeled ubiquitin, E1 and E2 (UbcH5b) as indicated. Polyubiquitination was determined by the detection of biotin-Ub.