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. 1998 Jun 23;95(13):7469–7474. doi: 10.1073/pnas.95.13.7469

Figure 1.

Figure 1

Detection of cell surface CD22 lectin activity on transfected CHO cells. Wild-type or CD22-transfected CHO cells were probed with the biotinylated polyacrylamide polymers in the presence or absence of inhibitors. Binding was detected using SA-PE and single-color flow cytometry. Negative control incubations had SA-PE alone. (A) Parental CHO-K1 incubated with and without 6′PAA-B; (B) CD22-CHO cells incubated with 6′PAA-B, mild periodate-treated (pt) 6′PAA-B, or 3′PAA-B; (C) competition of 6′PAA-B binding to CD22-CHO cells with a fivefold excess of nonbiotinylated 6′PAA or 3′PAA; (D) competition of 6′PAA-B to CD22-CHO cells with 1 mM α2–6-sialyllactose (Slac).