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Unmasking of 6′-sialyllactosamine lectin function in human PBMCs cells by sialidase or mild periodate treatment. PBMCs were washed well, preincubated in serum-free medium for 20 min at RT, and then treated with either AUS, mild periodate, or a buffer control (PBS) before being probed by 6′PAA-B followed by SA-PE.
