Skip to main content
. Author manuscript; available in PMC: 2009 Feb 1.
Published in final edited form as: Mol Cell Neurosci. 2007 Nov 1;37(2):376–387. doi: 10.1016/j.mcn.2007.10.014

Figure 1.

Figure 1

Membrane depolarization inhibits SGN neurite growth. A. SGN cultures were maintained in NT-3 (50 ng/ml), NT-3 with 30 mM [K+]o (30K) or NT-3 with 80 mM [K+]o (80K) for 48 h. Following fixation, the cultures were immunolabeled with anti-neurofilament 200 (NF-200) antibody followed by a fluorescently conjugated secondary antibody. Neurite length was determined by measuring the longest process extending from each SGN in 5–7 randomly selected fields/well. Results are presented as cumulative percent histograms where conditions with shorter neurites are shifted to the left compared with conditions with longer neurites. Each condition was performed in triplicate and repeated at least three times. n=cumulative number of SGNs scored for all repetitions. Each condition is significantly different (p<0.05) from the others by Kruskal-Wallis ANOVA on Ranks followed by a Dunn’s post-hoc comparison. B–D. Representative images of spiral ganglion cultures maintained in NT-3 (B), NT-3 with 30K (C), or NT-3 with 80K (D) for 48hr and labeled with anti-NF-200. Scale bar=100 μm.