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. 2008 Mar 10;180(5):957–971. doi: 10.1083/jcb.200708048

Figure 9.

Figure 9.

PTP1B is necessary for efficient invadopodia formation and invasion. (A) MTLn3 cells cultured on FN-coated glass coverslips were stained with anti-cortactin antibody and rhodamine phalloidin. Cells were cultured in the presence of vehicle or the cell-permeable PTP1B inhibitor CII. Quantification of invadopodia is expressed as the mean number of invadopodia per cell. Data are mean ± SEM of three independent experiments. *, P < 0.01 compared with control cells. (B) MTLn3 cells expressing control or PTP1Bsi were cultured on FN-coated glass coverslips and stained with anti-cortactin antibody and rhodamine phalloidin. Quantification of invadopodia is expressed as the mean number of invadopodia per cell. Data are mean ± SEM of three independent experiments. *, P < 0.01 compared with control cells. (C, left) MTLn3 cells were cultured in the presence of vehicle or CII and assayed for their ability to invade through the membrane. Invasion is shown relative to control cells. Data are mean ± SEM of three independent experiments. *, P < 0.05. (right) MTLn3 cells expressing control or PTP1B siRNA were plated on Matrigel-coated membranes and assayed for their ability to invade through the membrane. Invasion is shown relative to control cells. Data are mean ± SEM of three independent experiments. *, P < 0.02 compared with control cells. Bars: (A and B) 10 μm; (C) 1 mm.