Figure 6. Evaluation of footpad size (A and C) and Survival curves (B and D) of vaccinated B6 mice after M. ulcerans infection with 105 AFB in the right footpad.
Experiment 2 using MPL-A as adjuvant and 6 weeks as rest between vaccination and infection. Mice were vaccinated with empty vector (* n = 9), with 0.2 mg of BCG (▵ n = 10), with Ag85-DNA from M. ulcerans (□ n = 9), with DNA encoding 85A from M. tuberculosis (○ n = 10), with Ag85A DNA-M. ulcerans boosted with the Ag85A protein from M. ulcerans (▪ n = 9), or with DNA encoding Ag85A boosted with Ag85 protein from M. tuberculosis (• n = 10).