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. 2008 Mar 14;4(3):e1000017. doi: 10.1371/journal.pgen.1000017

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Lymphoblastoid cells from a FXS patient were treated with the indicated compounds as described in the Materials and Methods. Genomic DNA isolated from cells with and without treatment was tested for DNA methylation at the FMR1 promoter. The derivative of the dissociation curve of the bisulfite modified PCR fragment obtained from this procedure (dRFU/dT) was plotted as a function of temperature. The point of inflection corresponds to the Tm of the PCR product. Note that 2 peaks in the 5-aza-dC-treated samples are seen, one corresponding to completely demethylated alleles and a much smaller one, indicated by an asterisk, reflecting residual partially methylated alleles. RFU: relative fluorescent units.