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. 2008 Feb 29;4(2):e1000026. doi: 10.1371/journal.pcbi.1000026

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Chen et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) EMSA showing the specific binding of recombinant Drosophila Pum to radiolabeled hb NRE2 RNA. A total of 10 fmol of radiolabeled hb-NRE2 or control CRS RNA was used in each lane. Various amounts of recombinant GST-Pum or GST control proteins were used in different lanes: 2 fmol in lanes 2, 5, and 9; 10 fmol in lanes 3, 6, and 10; 50 fmol in lanes 4, 7, and 11; no protein in lanes 1 and 8. (B) EMSA showing the complex formed between Pum and hb-NRE2 at a higher molar ratio of protein to RNA. A total of 10 fmol of radiolabelled hb-NRE2 RNA was used in each lane. Recombinant GST-Pum proteins used in lanes 1, 2, 3, and 4 were 0 fmol, 50 fmol, 500 fmol, and 5 pmol, respectively.