Figure 5. In Vivo Confirmation of Predicted NRE Elements.

(A) Cartoon representations of the hb-transgene constructs and representative examples from cuticle preparations of corresponding transformant lines, showing normal/abnormal or rescued/partial rescued abdominal segmentation. Transgenic lines containing both hb NREs either in the normal context (wild type) or reinserted into the deletion construct (hb1,2) are fully regulated by Pum and yield embryos with the normal complement of 8 abdominal segments. Deletion of both of these NREs (hbΔ) prevents Pum-dependent translational repression leading to complete absence of abdominal segmentation. Insertion of either one hb NRE (hb2) or the predicted NRE from dlg1 (dlg1) are each sufficient to partially restore abdominal development. (B) The average numbers of abdominal segments are shown for each of four transformant lines resulting from each of the hb-transgene constructs. A total of 72–140 embryos of each transformant line were analyzed to count the number of abdominal segments to generate the mean number. (See Table S4 for frequency distribution of each line).