Table 1.

Anchorage-dependent tumorigenic properties of Rap1b-expressing cells

Exp.	Tumor formation, animals bearing tumors/animals injected		Soft agar assay, number of colonies/plate
	pL7-Hy	pL7-3-50	pL7-Hy	pL7-3-24	pL7-3-50
1	0/4	4/4	1	0	1
2	0/2	4/4	0	0	0

Ralp1b-expressing cells (pL7-3-50) induced tumor formation in all animals tested, whereas no tumor was observed for control (pL7-Hy) cells after 4 months of injection. For soft agar assays, cells were plated (5,000 cells per well in six-well dishes) in serum-containing medium and set on top of a 1% agar layer. Colonies (>50 cells) were scored under the microscope after 3 weeks of incubation.