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. 2008 Feb 29;4(2):e1000001. doi: 10.1371/journal.pgen.1000001

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Barberan-Soler and Zahler.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Gene model for AS of hrpf-1, two isoforms (a and b) are generated by either inclusion or skipping of exon 5; (B) Virtual gel of RT-PCR of hrpf-1 during C. elegans development as demonstrated on a Bioanalyzer (Agilent). The percentage of hrpf-1 messages that are spliced to include exon 5 are plotted; (C) RT-PCR of the hrpf-1 mRNA from total embryonic RNA from NMD defective worms (smg-1(r861)) compared to wild type embryo RNA. This experiment demonstrates that hrpf-1b is degraded by NMD; (D) regulation of alternative splicing of the C. briggsae hrpf-1 homolog (CBG04052) during development.