Skip to main content
. 2008 Mar 7;4(3):e1000013. doi: 10.1371/journal.pgen.1000013

Figure 4. GADD45A does not promote demethylation of the endogenous pOct4 promoter in NIH3T3 cells.

Figure 4

A. The transfection efficiency of NIH3T3 cells was analyzed with an EGFP expression vector and was calculated to be ∼60%. DIC, differential interference contrast image; GFP, green fluorescence. B. Schematic diagram of the mouse Oct4 promoter region. DE is the distal element, PE is the proximal element, and P is the minimal promoter of Oct4. Black triangles indicate HpaII sites and the open triangle shows a HhaI site. C and D. Bisulfite sequence analysis of all CpGs in the Oct4 upstream region. Closed circles are methylated CpGs and open circles are unmethylated CpGs. C, control vector transfected cells; D, GADD45A expression vector transfected cells.