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. 2008 Mar 21;4(3):e1000025. doi: 10.1371/journal.pgen.1000025

Figure 3. Analysis of hagfish MgRunxA and –B expression in different tissues of adult animals.

Figure 3

Quantification of MgRunxA and –B expression by qRT-PCR (A). Whereas MgRunxB was only weakly expressed in all tissues analyzed, MgRunxA showed a strong expression in calcified cartilage gills and soft cartilage. Expression of MgRunxA was also detected by radioactive in situ hybridisation in hard cartilage tissue (B, C). Insert of (B) is shown at higher magnification in (C) displaying the silver grains of the autoradiography emulsion indicating MgRunxA expression. B: Brain, C-h: Hard cartilage, C-s: Soft cartilage, Cho: Chorda, G: Gills, Gb: Gall bladder, G-a: Anterior gut, G-m: Midgut, G-h: Hindgut, H: Heart, L: Liver, Mu: Muscle, Sk: Skin.