Table 3. Functional annotation proposed for 16 Esv-mutants published by smith and colleagues [64].

Gene labela	Gene Name	Esv-mutant Namea	Annotation	Putative Ortholog	Essential in A. baylyi ADP1b	Essential in E. coli c
A1S_3223	-	EsvA	putative transcriptional regulator		No Homolog	No Homolog
A1S_1232	-	EsvB	putative transcriptional regulator	ABAYE2526	No Homolog	No Homolog
A1S_1012	ureA	EsvC	urease gamma subunit	ABAYE2778,ABSDF2377,ACIAD1089	No	No
A1S_2447	pstC	EsvD	high-affinity phosphate transport protein	ABAYE1033,ABSDF1101,ACIAD1213	No	No
A1S_2313	queF	EsvE1	7-cyano-7-deazaguanine reductase	ABAYE1164,ABSDF1213,ACIAD2261	No	No
A1S_2314	-	EsvE2	conserved hypothetical protein	ABAYE1163,ABSDF1212,ACIAD2262	No	No
A1S_2315	rodA	EsvE3	rod shape-determining protein	ABAYE1162,ABSDF1211,ACIAD2263	No	Yes
A1S_3218	czcB	EsvF1	RND divalent metal cation efflux membrane fusion protein	ABAYE0270,ABSDF0264,ACIAD3376	No	No
A1S_3290	-	EsvF2	putative transcriptional regulator	ABAYE0200,ABSDF3398,ACIAD3452	No	No
A1S_0378	etfD	EsvG	electron transfer flavoprotein-ubiquinone oxidoreductase	ABAYE3397,ABSDF3133,ACIAD3259	No	No
A1S_2262	rpoH	EsvH	sigma H (sigma 32) factor of RNA polymerase	ABAYE1217,ABSDF1264,ACIAD1311	Yes	Yes
A1S_2037	-	EsvI	putative transcriptional regulator		No Homolog	No Homolog
A1S_3329	-	EsvJ	conserved hypothetical protein	ABAYE0156,ABSDF3464,ACIAD3508	No	No
A1S_1586	-	EsvK1	putative phage endonuclease		No Homolog	No Homolog
A1S_1587	-	EsvK2	putative phage Terminase		No Homolog	No Homolog
A1S_0310	uvrC	EsvL	excinuclease ABC subunit C	ABAYE3465,ABSDF3216,ACIAD0340	No	No

Automatic functional annotation was first generated using our annotation pipeline [71] subsequently reviewed by human expert.

^ataken from [64],

^btaken from [36],

^cdata from E. coli chromosome (PEC) database (http://www.shigen.nig.ac.jp/ecoli/pec/index.jsp).