Figure 3.

Treatment with the ILK inhibitor QLT-0267 causes apoptotic-independent effects on mitotic spindles and an accumulation of cells in mitosis. (A) HEK293 cells were treated with increasing concentrations of QLT-0267 for 7 h and the mitotic index was calculated. Data are mean ± SD of three independent experiments in which >400 cells were counted for each condition. (B and C) Cells were treated with QLT-0267 for 7 h and stained as indicated. (D) Cells were treated with the MEK inhibitor PD098059. (E and F) HeLa cells were treated with QLT-0267 for 1 h (E) or QLT-0267 + Z-VAD-FMK for 7 h (F). Bars, ∼10 μm. (G) Quantification of apoptosis in indicated samples by Cell Death Detection ELISA PLUS. All samples, except QLT-0267 5 μm 1 h, are from the 7-h time point. Data are mean ± SD from two experiments. (H) Indicated cell lysates (all 7-h treatments) were Western blotted for PARP.