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. Author manuscript; available in PMC: 2008 Mar 7.
Published in final edited form as: Biochemistry. 2007 Mar 23;46(16):4876–4887. doi: 10.1021/bi061651w

Figure 5.

Figure 5

Perrin plots for the quenching-resolved emission anisotropy of tyrosine fluorescence of TBP and TBPc (excitation at 275 nm and emission at 306 nm) by acrylamide (A) and iodide (B): free protein at 6 μM (■) and TBP–DNA complex at 6 μM (○). The data for the TBP–DNA complex are not shown in panel B since its tyrosine fluorescence is inaccessible to iodide. The parameter A is the fluorescence anisotropy (eq 3, Experimental Procedures); F0 and F are defined in the legend of Figure 4.