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. Author manuscript; available in PMC: 2008 Mar 7.
Published in final edited form as: Biochemistry. 2007 Mar 23;46(16):4876–4887. doi: 10.1021/bi061651w

Figure 6.

Figure 6

Stern–Volmer plots (A and B) and Perrin plots (C and D) for the quenching of tryptophan fluorescence (excitation at 275 nm and emission at 365 nm) of TBP by acrylamide (A and C) and iodide (B and D). The straight lines represent the best fit to eqs 1 and 3. Conditions: TBP at 1 (□) and 6 μM (△) and TBP at 6 μM complexed with a 1.2-fold molar excess of DNA bearing the sequence TATAAAAG (●) or N-acetyl-L-tryptophan ethyl ester (▼). The parameters A, F0, and F are the same as in Figures 4 and 5.